ABSTRACT
Abstract The aim of this study was to describe the initial oral microbiota and how delivery mode and feeding practices impact its diversity in 0-2-month-old infants. This was a cross-sectional study that consisted of one collection of saliva samples from 0-2-month infants at baseline. Ten pairs of mothers and infants were selected. Medical health history, pregnancy, birth, feeding practices (breastfeeding or milk formula), and infant health status was obtained. Pooled microbial samples were obtained from the oral surfaces using a sterile cotton swab. Infants did not receive any breast milk before sampling. After collection, each swab was analyzed through microbiological culture-based procedures, using selective mediums. Cultures were analyzed for the presence of Streptococci, Lactobacillus, Staphylococcus, Enterobacterium , and Candida albicans . Twenty percent of the samples were serially diluted (10-2) to assess the number of bacteria expressed as CFU. Bacillota was the leading phylogenetic group in the infant's pooled microbial sample. The most prevalent genera were Streptococcus, Lactobacillus , and Staphylococcus . Two participants had a positive growth of Candida albicans . The association between genus group, type of delivery, and feeding practices was not statistically significant (p > 0.05). Lactobacillus genus was frequently present in the cesarean delivery group but with slightly higher counts in a vaginal delivery study subject. Exclusively breastfed infants showed presence of Streptococcus, Lactobacillus, Staphylococcus . The oral microbiome in infants (0-2 month-old) is highly heterogeneous and dynamic. Microbiota composition seems to be impacted by mode of delivery, with slight differences among groups. Breastmilk appears as an essential factor in maintaining the oral microbiome's stability and diversity.
ABSTRACT
Introduction: Wilms' tumor is a malignant renal neoplasm that frequently occurs in children during the first decade of life. clinically, it is a rapidly growing abdominal mass that causes low back pain and hematuria. computerized axial tomography or nuclear magnetic resonance are fundamental for its diagnosis, and chemotherapy and surgery have become first-choice treatments. after diagnosis, the majority of treatment plans involve the administration of antineoplastic drugs, whose side effects may include mucositis, candidiasis, xerostomia, caries, and worsen other previously diagnosed lesions, regardless of the organ affected by the tumor. treatment is more effective if provided by a multidisciplinary team in which the dentist plays a significant role in the implementation of an integral oral care protocol. in the present study, the management of a pediatric patient under antineoplastic treatment for Wilms' tumor is reported. case report: a four-year-old female patient diagnosed with Wilms' tumor, who required antineoplastic treatment. she had temporary dentition with early childhood caries, irreversible pulpal lesions and agenesis of teeth 72, 82, and the germ of tooth 42. the patient received modeling based behavior management therapy, prophylactic oral hygiene, and restoration of teeth affected by caries. to present this case, the "CARE" guidelines were used. conclusion: poor oral health status prior to cancer therapy directly affects the quality of life and the treatment of a patient, increasing the risks of local or systemic infections. as such evaluation and dental treatment before antineoplastic therapy is important to prevent oral complications and lesions.